Titolo | BCR-ABL prevents c-Jun-mediated and proteasome-dependent FUS (TLS) proteolysis through a protein kinase CβII-dependent pathway |
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Tipo di pubblicazione | Articolo su Rivista peer-reviewed |
Anno di Pubblicazione | 2000 |
Autori | Perrotti, D., Iervolino A., Cesi Vincenzo, Cirinna M., Lombardini S., Grassilli E., Bonatti S., Claudio P.P., and Calabretia B. |
Rivista | Molecular and Cellular Biology |
Volume | 20 |
Paginazione | 6159-6169 |
ISSN | 02707306 |
Parole chiave | amino acid substitution, animal cell, Animalia, Animals, Apoptosis, article, BCR ABL protein, bcr-abl, Cell Line, controlled study, Cysteine Endopeptidases, DNA binding, enzyme activation, enzyme phosphorylation, Fusion proteins, gene control, Gene expression, gene induction, gene repression, gene translocation, Genes, heterogeneous nuclear ribonucleoprotein, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Heterogeneous-Nuclear Ribonucleoproteins, jun, Mice, Multienzyme Complexes, nonhuman, Phosphorylation, priority journal, proteasome, Proteasome Endopeptidase Complex, protein degradation, Protein Kinase C, protein processing, protein protein interaction, Ribonucleoproteins, RNA-Binding Protein FUS, signal transduction, stress activated protein kinase, transactivation |
Abstract | The DNA binding activity of FUS (also tmown as TLS), a nuclear pro-oncogene involved in multiple translocations, is regulated by BCR-ABL in a protein ldnase CβII (PKCβII)-dependent manner. We show here that in normal myeloid progenitor cells FUS, although not visibly ubiquitinated, undergoes proteasome-dependent degradation, whereas in BCR-ABL-expressing cells, degradation is suppressed by PKCβII phosphorylation. Replacement of serlne 256 with the phosphomimetic aspartic acid prevents proteasome-dependent proteolysis of FUS, while the serine-256-to-alanine FUS mutant is unstable and susceptible to degradation. Ectopic expression of the phosphomimetic S256D FUS mutant in granulocyte colony-stimulating factor-treated 32Dcl3 cells induces massive apoptosis and inhibits the differentiation of the cells escaping cell death, while the degradation-prone S256A mutant has no effect on either survival or differentiation. FUS proteolysis is induced by c-Jun, is suppressed by BCR-ABL or Jun kinase 1, and does not depend on c-Jun transactivation potential, ubiquitination, or its interaction with Jun kinase 1. In addition, c-Jun-induced FUS proteasome-dependent degradation is enhanced by heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and depends on the formation of a FUS-Jun-hnRNP A1-containing complex and on lack of PKCβII phosphorylation at serine 256 but not on FUS ubiquitination. Thus, novel mechanisms appear to be involved in the degradation of FUS in normal myeloid cells; moreover, the ability of the BCR-ABL oncoprotein to suppress FUS degradation by the induction of posttranslational modifications might contribute to the phenotype of BCR-ABL-expressing hematopoietic cells. |
Note | cited By 53 |
URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033858675&doi=10.1128%2fMCB.20.16.6159-6169.2000&partnerID=40&md5=9a90c0156de071e46ddf0e3251cf219b |
DOI | 10.1128/MCB.20.16.6159-6169.2000 |
Citation Key | Perrotti20006159 |