In a recent paper [P. Rizzo, G. Bordoni, A. Marzani, and J. Vipperman, "Localization of Sound Sources by Means of Unidirectional Microphones, Meas. Sci. Tech., 20, 055202 (12pp), 2009] the proof-of-concept of an approach for the localization of acoustic sources was presented. The method relies on the use of unidirectional microphones and amplitude-based signals' features to extract information about the direction of the incoming sound. By intersecting the directions identified by a pair of microphones, the position of the emitting source can be identified. In this paper we expand the work presented previously by assessing the effectiveness of the approach for the localization of an acoustic source in an indoor setting. As the method relies on the accurate knowledge of the microphones directivity, analytical expression of the acoustic sensors polar pattern were derived by testing them in an anechoic chamber. Then an experiment was conducted in an empty laboratory by using an array of three unidirectional microphones. The ability to locate the position of a commercial speaker placed at different positions in the room is discussed. The objective of this study is to propose a valid alternative to the common application of spaced arrays and therefore to introduce a new generation of reduced size sound detectors and localizers. The ability of the proposed methodology to locate the position of a commercial speaker placed at different positions in the room was evaluated and compared to the accuracy provided by a conventional time delay estimate algorithm. © 2011 SPIE.