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Molecular characterization of the anthocyanidin synthase gene in Forsythia x intermedia reveals organ-specific expression during flower development

TitoloMolecular characterization of the anthocyanidin synthase gene in Forsythia x intermedia reveals organ-specific expression during flower development
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione1999
AutoriRosati, C., Cadic A., Duron M., Ingouff M., and Simoneau P.
RivistaPlant Science
Volume149
Paginazione73-79
ISSN01689452
Parole chiaveAnthocyanin, article, enzyme activity, Forsythia, gene activity, Gene expression, genetic analysis, nonhuman, promoter region, protein blood level, protein DNA binding, reverse transcription polymerase chain reaction, structural gene
Abstract

The study of the anthocyanidin synthase (ANS) gene, one of the late structural genes in the anthocyanin pathway, was undertaken in Forsythia x intermedia cv. 'Spring Glory'. Previous molecular and biochemical studies had demonstrated expression and activity of genes and enzymes upstream of ans. The ans gene was cloned and shown to be present in two copies in the Forsythia genome. Expression analyses carried out on flower organs showed that ans was expressed exclusively in anthocyanin-containing sepals and not in anthocyaninless anthers and petals. ans expression in sepals showed induction of transcription at early flower developmental stages. Inspection of the ans promoter region as far as 790 bp upstream of the start codon revealed several potential DNA-protein binding motifs. The results from this paper, combined with previous data, show that the lack of ans expression should be the major cause of the absence of anthocyanins in Forsythia petals, thus providing directions for genetic engineering of flower color in this ornamental species.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0033550155&doi=10.1016%2fS0168-9452%2899%2900146-6&partnerID=40&md5=4320395abeccffae757482fb51b5f8ad
DOI10.1016/S0168-9452(99)00146-6
Citation KeyRosati199973