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Metabolic engineering of xanthophyll content in tomato fruits

TitoloMetabolic engineering of xanthophyll content in tomato fruits
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2002
AutoriDharmapuri, S., Rosati C., Pallara Patrizia, Aquilani R., Bouvier F., Camara B., and Giuliano Giovanni
RivistaFEBS Letters
Volume519
Paginazione30-34
ISSN00145793
Parole chiaveArabidopsis, article, beta carotene, beta carotene hydroxylase, beta cryptoxanthin, Blotting, carotene, Carotenoid, cryptoxanthin, Dominant, enzyme assay, fruit, fruit ripening, Gene expression, Genes, Genetic engineering, Genetically Modified, Intramolecular Lyases, lycopene, lycopene beta cyclase, Lycopersicon esculentum, metabolic engineering, Mixed Function Oxygenases, Phenotype, plant leaf, Plant leaves, Plants, priority journal, promoter region, protein expression, Reverse Transcriptase Polymerase Chain Reaction, reverse transcription polymerase chain reaction, Tomato, transgene, Transgenes, unclassified drug, Western, Western blotting, xanthophyll, Xanthophylls, Zeaxanthin
Abstract

Ripe tomato fruits accumulate significant amounts of the linear carotene lycopene, but only trace amounts of xanthophylls (oxygenated carotenoids). We overexpressed the lycopene β-cyclase (b-Lcy) and β-carotene hydroxylase (b-Chy) genes under the control of the fruit-specific Pds promoter. Transgene and protein expression was followed through semi-quantitative reverse transcription-PCR, Western blotting, and enzyme assays. Fruits of the transformants showed a significant increase of β-carotene, β-cryptoxanthin and zeaxanthin. The carotenoid composition of leaves remained unaltered. The transgenes and the phenotype are inherited in a dominant Mendelian fashion. This is the first example of successful metabolic engineering of xanthophyll content in tomato fruits. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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cited By 86

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0037157193&doi=10.1016%2fS0014-5793%2802%2902699-6&partnerID=40&md5=f866f7e5ccd71eedd16de2ba646bc0e6
DOI10.1016/S0014-5793(02)02699-6
Citation KeyDharmapuri200230