We exploited the gentleness of the microcontact printing (μCP) technique to pattern stripes or grids of physisorbed Laminin-1 (Ln-1) onto underivatized glass. We verified the effect of this lithographic technique on the conformation of the laminin molecules and on the morphology of the protein matrices obtained from different concentrations and with or without calcium. Firstly we investigated the obtained pattern by indirect immunofluorescence, which both showed a well-defined printed area and gave a preliminary indication of protein structural integrity. Then, we imaged the patterned regions by Atomic Force Microscopy (AFM), demonstrating both features of physiological matrices, including correct interactions between the protein arms, and recognizable single Ln-1 molecules. Interestingly this result was strongly affected by the composition of the protein solution.