Titolo | Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein |
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Tipo di pubblicazione | Articolo su Rivista peer-reviewed |
Anno di Pubblicazione | 1997 |
Autori | Crescenzi, A., d'Aquino Luigi, Nuzzaci M., Ostuni A., Bavoso A., Comes S., De Stradis A., and Piazzolla P. |
Rivista | Journal of Virological Methods |
Volume | 69 |
Paginazione | 181-189 |
ISSN | 01660934 |
Parole chiave | Amino Acid Sequence, Antibodies, Antibody, article, Blotting, Capsid, Capsid Proteins, Cloning, coat protein, controlled study, Enzyme-Linked Immunosorbent Assay, Immunoelectron, Microscopy, Molecular, Molecular Sequence Data, Mosaic virus, nonhuman, nucleotide sequence, Peptide Fragments, Plant Diseases, Plant extracts, plant virus, Plum pox virus, polyclonal antibody, Potyvirus, priority journal, Prunus avium, Prunus domestica, RNA viruses, Sequence Analysis, Species Specificity, synthetic peptide, Viral, virus characterization, virus detection, virus particle, virus protein, Western |
Abstract | Comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (PPV-SwC) with the corresponding regions of several other PPV strains indicated that the main differences are in the N-terminal region. Polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of tile N-terminal region of PPV-SwC coat protein. They specifically detected PPV-SwC in different immunochemical tests. |
Note | cited By 15 |
URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031443134&doi=10.1016%2fS0166-0934%2897%2900158-4&partnerID=40&md5=11ab2ff3cefdf67fa1cf0372380c484b |
DOI | 10.1016/S0166-0934(97)00158-4 |
Citation Key | Crescenzi1997181 |