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Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein

TitoloProduction of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione1997
AutoriCrescenzi, A., d'Aquino Luigi, Nuzzaci M., Ostuni A., Bavoso A., Comes S., De Stradis A., and Piazzolla P.
RivistaJournal of Virological Methods
Volume69
Paginazione181-189
ISSN01660934
Parole chiaveAmino Acid Sequence, Antibodies, Antibody, article, Blotting, Capsid, Capsid Proteins, Cloning, coat protein, controlled study, Enzyme-Linked Immunosorbent Assay, Immunoelectron, Microscopy, Molecular, Molecular Sequence Data, Mosaic virus, nonhuman, nucleotide sequence, Peptide Fragments, Plant Diseases, Plant extracts, plant virus, Plum pox virus, polyclonal antibody, Potyvirus, priority journal, Prunus avium, Prunus domestica, RNA viruses, Sequence Analysis, Species Specificity, synthetic peptide, Viral, virus characterization, virus detection, virus particle, virus protein, Western
Abstract

Comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (PPV-SwC) with the corresponding regions of several other PPV strains indicated that the main differences are in the N-terminal region. Polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of tile N-terminal region of PPV-SwC coat protein. They specifically detected PPV-SwC in different immunochemical tests.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0031443134&doi=10.1016%2fS0166-0934%2897%2900158-4&partnerID=40&md5=11ab2ff3cefdf67fa1cf0372380c484b
DOI10.1016/S0166-0934(97)00158-4
Citation KeyCrescenzi1997181