Titolo | Thermodynamic study of Langmuir antibody films for application to immunosensors |
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Tipo di pubblicazione | Articolo su Rivista peer-reviewed |
Anno di Pubblicazione | 1991 |
Autori | Ahluwalia, A., De Rossi D., Monici M., and Schirone Antonio |
Rivista | Biosensors and Bioelectronics |
Volume | 6 |
Paginazione | 133-141 |
ISSN | 09565663 |
Parole chiave | Antibodies–Thermodynamics, Antibody, article, Biosensor, Biosensors–Design, Films–Langmuir-Blodgett, fluorescence, Immunoglobulin, immunoglobulin G, immunology, Immunosensors, Langmuir Antibody Films, priority journal, Thermodynamics–Calculations, Total Internal Reflection Fluorescence (TIRF) |
Abstract | The thermodynamic functions, compressibility, entropy and free energy of desorption have been derived from a set of π-A isotherms of human immunoglobulin G films at an air-water interface. Criteria for optimum filmlift conditions have been obtained from an analysis of these functions. By the application of a basic model, a picture of the desorption behaviour of segments of the antibodies has also been constructed. The results of this study have been used to estimate the theoretical sensing limit of a TIRF based immunosensor. © 1991. |
Note | cited By 35 |
URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025805893&doi=10.1016%2f0956-5663%2891%2987037-C&partnerID=40&md5=4aadc3fc85e09dc1c681f1b6fea0f628 |
DOI | 10.1016/0956-5663(91)87037-C |
Citation Key | Ahluwalia1991133 |