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Role of mRNA stability in the different patterns of cytokine production by CD4+ cells from young and old mice

TitoloRole of mRNA stability in the different patterns of cytokine production by CD4+ cells from young and old mice
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione1998
AutoriPioli, Claudio, Pucci S., Barile S., Frasca D., and Doria G.
RivistaImmunology
Volume94
Paginazione380-387
ISSN00192805
Parole chiaveAging, animal cell, animal experiment, Animals, Antibodies, Antigens, article, CD28, CD3, CD4, CD4 antigen, Cell Division, Cells, Cultured, Cytokines, enzyme linked immunosorbent assay, gamma interferon, genetic stability, immune system, Inbred C57BL, Interferon Type II, interleukin 10, interleukin 2, interleukin 4, Interleukin-10, Interleukin-2, Interleukin-4, lymphocyte proliferation, male, Messenger, Mice, Monoclonal, mouse, nonhuman, Polymerase Chain Reaction, priority journal, RNA, T lymphocyte subpopulation, T-Lymphocytes
Abstract

CD4+ cells from young (3 months) and old (19 months) mice were stimulated by plate-bound anti-CD3 monoclonal antibody (mAb) alone or also by soluble anti-CD28 mAb. Supernatants were analysed by enzyme-linked immunosorbent assay (ELISA) to determine cytokine concentrations. Total RNA was extracted from cells, reverse transcribed and the cDNA amplified by polymerase chain reaction (PCR) to evaluate the amount of specific mRNA. The results indicate that anti-CD3 alone is not sufficient to induce interleukin- 2 (IL-2) production in CD4+ cells from both young and old mice. However, anti-CD28, together with anti-CD3 mAb, induces a much higher production of IL-2 in CD4+ cells from young as compared with old mice. Conversely, interferon-γ (IFN-γ) production is also induced by anti-CD3 alone and is higher in CD4+ cells from old as compared with young mice. Upon addition of anti-CD28 mAb, IFN-γ production increases in both groups, but it remains much higher in old than in young mice. Also the production of IL-4 and IL-10 is induced by anti-CD3 mAb but it is increased by the addition of anti-CD28 mAb. CD4+ cells from old mice produce more IL-4 and IL-10 as compared with cells from young mice. The amounts of cytokine specific mRNA in CD4+ cells from young and old mice parallel the cytokine levels in culture supernatants. Results on the mRNA turnover indicate that when CD4+ cells are stimulated by anti-CD3 or costimulated also by anti-CD28 mAb, the IFN-γ, IL-4 and IL-10 specific mRNAs are more stable in old than in young mice, suggesting that mRNA stability has a relevant role in the different patterns of cytokine production.

Note

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0031822064&doi=10.1046%2fj.1365-2567.1998.00523.x&partnerID=40&md5=7377a6bc9fbed57464d9ec763b7357ab
DOI10.1046/j.1365-2567.1998.00523.x
Citation KeyPioli1998380