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Development and characterization of microsatellite markers from chromosome 1-specific DNA libraries of Vicia faba

TitoloDevelopment and characterization of microsatellite markers from chromosome 1-specific DNA libraries of Vicia faba
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2002
AutoriPožárková, D., Koblížková A., Román B., Torres A.M., Lucretti Sergio, Lysák M.A., Doležel Jaroslav, and Macas J.
RivistaBiologia Plantarum
Volume45
Paginazione337 - 345
Data di pubblicazione2002///
Parole chiaveField bean, Flow-sorting, Genetic map, Physical mapping, Plant chromosomes
Abstract

An integrated approach has been developed for targeted retrieval of microsatellite markers from selected regions of the field bean (Vicia faba L.) genome. The procedure is based on a combination of advanced physical and genetic mapping techniques and includes the following steps: 1) flow-sorting of metaphase chromosomes, 2) construction of microsatellite-enriched chromosome-specific DNA libraries, 3) isolation of polymorphic microsatellite sequences from the libraries, 4) testing chromosome specificity of the microsatellites using polymerase chain reaction with purified fractions of individual chromosome types, and 5) integration of chromosome-specific markers into a genetic map. Several strategies for isolation of microsatellite clones were tested, including direct screening of non-enriched libraries with single or mixed probes and screening of the libraries after one or two rounds of enrichment. Finally, the usefulness of this approach was demonstrated by the retrieval of novel markers from a selected portion of the largest field bean chromosome (No. 1).

Note

Cited By (since 1996): 26Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0036081369&partnerID=40&md5=c4c8129b08c2acd5fa3e2e8273fcad6d
Citation Key395