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Effects of 50 Hz Electromagnetic Field Exposure on Apoptosis and Differentiation in a Neuroblastoma Cell Line

TitoloEffects of 50 Hz Electromagnetic Field Exposure on Apoptosis and Differentiation in a Neuroblastoma Cell Line
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2003
AutoriPirozzoli, M.C., Marino Carmela, Lovisolo G.A., Laconi C., Mosiello Lucia, and Negroni Anna
RivistaBioelectromagnetics
Volume24
Paginazione510 - 516
Data di pubblicazione2003///
Parole chiaveCamptothecin, LAN-5, Oncogene expression, Proliferation, Retinoic acid
Abstract

Experiments were carried out to assess whether a magnetic field of 50 Hz and 1 mT can influence apoptosis and proliferation in the human neuroblastoma cell line LAN-5. TUNEL assays and poly-ADP ribose polymerase (PARP) expression analysis were performed to test apoptosis induction, and the WST-1 assay was used to calculate the proliferation index in a long term exposure. No alterations were found in cellular ability to undergo programmed cell death, but a small increase in the proliferation index was evidenced after 7 days of continuous exposure. Also, a slight and transient increase of B-myb oncogene expression was detected after 5 days of exposure. Combined exposures of cells to EMF and to chemical agents which interfere with proliferation, such as the differentiative agent retinoic acid and the apoptotic inducer camptothecin, showed an antagonistic effect of magnetic fields against the differentiation of the LAN-5 cells and a protective effect towards apoptosis. © 2003 Wiley-Liss, Inc.

Note

Cited By (since 1996): 22Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-1242269143&partnerID=40&md5=09fb200c0f78bc9f9b1a5e67bfa2f160
Citation Key383