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Tomato yellow leaf curl Sardinia virus can overcome transgene-mediated RNA silencing of two essential viral genes

TitoloTomato yellow leaf curl Sardinia virus can overcome transgene-mediated RNA silencing of two essential viral genes
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2004
AutoriNoris, Emanuela, Lucioli Alessandra, Tavazza Raffaela, Caciagli P., Accotto Gian Paolo, and Tavazza Mario
RivistaJournal of General Virology
Volume85
Paginazione1745 - 1749
Data di pubblicazione2004///
Abstract

To evaluate RNA silencing for the control of geminivirus infection, two classes of post-transcriptionally silenced (PTS) plants were tested using Tomato yellow leaf curl Sardinia virus (TYLCSV) Rep-210-transgenic plants, a sense × antisense hybrid and two multicopy sense lines. In both classes, PTS plants accumulated low or undetectable amounts of Rep-210 protein and mRNA but high amounts of Rep-210 small interfering RNAs. PTS plants were susceptible to TYLCSV when challenged by agroinoculation or using high viruliferous whitefly (Bemisia tabaci) pressure, although some plants were resistant at low whitefly pressure. Delayed infections were also observed, indicating that TYLCSV could overcome transgene silencing of rep and of the nested C4 gene. TYLCSV infection boosted transgene silencing but this did not lead to recovery. The data suggest that if the virus reaches a threshold level of expression/replication in the initially infected cells then virus spreading can no longer be prevented. © 2004 SGM.

Note

Cited By (since 1996): 16Export Date: 25 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-2942648484&partnerID=40&md5=51f848e5dfb5e90a2cf4908870ec1ab6
Citation Key284