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Molecular characterization and in planta detection of Fusarium moniliforme endopolygalacturonase isoforms

TitoloMolecular characterization and in planta detection of Fusarium moniliforme endopolygalacturonase isoforms
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2001
AutoriDaroda, Lorenza, Hahn K., Pashkoulov D., and Benvenuto Eugenio
RivistaPhysiological and Molecular Plant Pathology
Volume59
Paginazione317 - 325
Data di pubblicazione2001///
Parole chiaveEndopolygalacturonase, Fusarium moniliforme, Monoclonal antibody, Zea mays
Abstract

The activation of Fusarium moniliforme endopolygalacturonase (endoPG) was studied during infection of maize plants. EndoPG is a plant cell wall degrading enzyme that cleaves the pectin component causing cell death. The authors generated several hybridoma cell lines producing endoPG specific monoclonal antibodies. One monoclonal antibody was selected and successfully used in Western blotting analysis to detect F. moniliforme endoPG secretion in vitro and in planta. Two F. moniliforme strains (FC-10 and 62264) were used for the studies. Both strains revealed the expression of a single endoPG in vitro as in planta. EndoPG from strain FC-10 presented four isoforms whereas only two isoforms were revealed in the endoPG from strain 62264. Differences were also found in the sequences of the two endoPG genes indicating the presence of endoPG variability among F. moniliforme strains. © 2001 Elsevier Science Limited.

Note

Cited By (since 1996): 6Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0035721655&partnerID=40&md5=80115fc59cee7156138cd53c0a04ecb7
Citation Key404