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Investigation on metabolic fingerprint diversity of different Agrobacterium strains and cereal transformation

TitleInvestigation on metabolic fingerprint diversity of different Agrobacterium strains and cereal transformation
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2007
AuthorsLatini, Arianna, Tasso Flavia, Sperandei Maria, Morelli F., Pugnali Margherita, Dettori Marco, Cantale Cristina, and Galeffi Patrizia
JournalJournal of Genetics and Breeding
Volume61
Pagination143 - 150
Date Published2007///
KeywordsAgrobacterium tumefaciens, Biolog microstation, Calli regeneration, Cereal transformation, Metabolic fingerprint, Triticale, Triticum aestivum
Abstract

Many factors affect the Agrobacterium-mediated transformation of monocotyledons as plant genotype, Agrobacterium strain and binary vectors. In order to find the optimal conditions for accomplishing the cereal transformation by Agrobacterium we compared the metabolic fingerprinting of different bacterial strains, obtained by the Biolog system, in view to highlight potential correlation between their metabolic diversity and their transformation abilities. Two Agrobacterium strains, representative for the octopine and nopaline groups, have been tested to compare their transformation capability in two dicotyledonous species and in cereals. In addition, the regenerative abilities of various Triticum aestivum L. and Triticale varieties were also tested with the final aim of finding the better conditions for the generation of the wheat transgenic plants.

Notes

Export Date: 28 January 2011Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-78149445780&partnerID=40&md5=8f4caa4d109dfcb4e69c882956ca0744
Citation Key1052