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Conclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams

TitleConclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2011
AuthorsBussotti, F., Pollastrini M., Cascio C., Desotgiu R., Gerosa G., Marzuoli R., Nali C., Lorenzini G., Pellegrini E., Carucci M.G., Salvatori Elisabetta, Fusaro L., Piccotto M., Malaspina P., Manfredi A., Roccotello E., Toscano S., Gottardini E., Cristofori A., Fini A., Weber D., Baldassarre V., Barbanti L., Monti A., and Strasser R.J.
JournalEnvironmental and Experimental Botany
Volume73
Pagination116-119
ISSN00988472
KeywordsCalibration, Chlorophyll, comparative study, correlation, data set, fluorescence, instrumentation, Italy, leaf area, measurement method, Reaction kinetics, reliability analysis, research work, Standardization
Abstract

Two field exercises were carried out to compare chlorophyll a fluorescence measurements taken in the field by field teams working on the same project. In the first exercise (2007, Passo Pura, Ampezzo, Udine, Northern Italy) the operators took measurements on the same leaf areas (maintaining fixed leaf clips); in the second (2009, Monterotondo Marittimo, Grosseto, Central Italy) the teams worked independently, but addressing a common research question. The results of the first exercise showed that: (a) FV/FM was stable and had little variation among teams and instruments; (b) the results from the different teams correlated well; (c) the most suitable parameters of fast kinetics analysis are those measured on the normalized fluorescence transients. In the second exercise, when the teams worked independently, the results were much more variable and the correlations between measurements of different operators were weak. These results suggest that field chlorophyll a fluorescence measurements taken by different teams/operators can be comparable only if particular care is taken to the internal variability of the samples and a standardized sampling strategy is applied. A statistically sound representation of a population can be then reached. © 2010 Elsevier B.V.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-80052439194&doi=10.1016%2fj.envexpbot.2010.10.023&partnerID=40&md5=8738196b3308626b4aafe3bda351ee96
DOI10.1016/j.envexpbot.2010.10.023
Citation KeyBussotti2011116