Title | Leptin induction following irradiation is a conserved feature in mammalian epithelial cells and tissues |
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Publication Type | Articolo su Rivista peer-reviewed |
Year of Publication | 2017 |
Authors | Licursi, V., M. Guidi Cestelli, Del Vecchio G., Mannironi C., Presutti C., Amendola R., and Negri R. |
Journal | International Journal of Radiation Biology |
Volume | 93 |
Pagination | 947-957 |
ISSN | 09553002 |
Keywords | amylin, animal, animal cell, animal experiment, animal model, animal tissue, Animals, article, biosynthesis, Breast cancer, cancer radiotherapy, cancer tissue, cell culture, Cells, clinical evaluation, clinical feature, controlled study, Cultured, dentin matrix protein 1, dose response, Dose-Response Relationship, Drug, Epidermis, Epithelial Cells, epithelium, epithelium cell, GAGE12D protein, GCG protein, HTR3A protein, human, human cell, human tissue, Humans, IBSP protein, IFITM5 protein, LECT1 protein, Leptin, major clinical study, male, mammal cell, matrix extracellular phosphoglycoprotein, messenger RNA, metabolic regulation, metabolism, Mice, microarray analysis, microRNA, microRNA 125b 3p, microRNA 206, microRNA 22, microRNA 294, microRNA 294 5p, microRNA 29a 3p, microRNA 29b, microRNA 29b 3p, microRNA 29c 3p, microRNA 470, mouse, neutron radiation, nonhuman, osteogenin, physiology, priority journal, PROL1 protein, Protein, protein expression, Radiation Dosage, radiation dose, radiation response, real time polymerase chain reaction, skin epithelium, SMR3B protein, subcutaneous fat, transcription regulation, transcriptome, TTR protein, UGT2B4 protein, unclassified drug |
Abstract | Purpose: Leptin (LEP) is a peptide hormone with multiple physiological functions. Besides its systemic actions, it has important peripheral roles such as a mitogen action on keratinocytes following skin lesions. We previously showed that LEP mRNA is significantly induced in response to neutron irradiation in mouse skin and that the protein increases in the irradiated epidermis and in the related subcutaneous adipose tissue. In this work, we investigated the post-transcriptional regulation of LEP by miRNAs and the conservation of LEP’s role in radiation response in human cells. Methods: We used microarray analysis and real-time polymerase chain reaction (RT-PCR) to analyze modulation of miRNAs potentially targeting LEP in mouse skin following irradiation and bioinformatic analysis of transcriptome of irradiated human cell lines and cancer tissues from radiotherapy-treated patients to evaluate LEP expression. Results and conclusions: We show that a network of miRNAs potentially targeting LEP mRNA is modulated in irradiated mouse skin and that LEP itself is significantly modulated by irradiation in human epithelial cell lines and in breast cancer tissues from radiotherapy-treated patients. These results confirm and extend the previous evidence that LEP has a general and important role in the response of mammalian cells to irradiation. © 2017 Informa UK Limited, trading as Taylor & Francis Group. |
Notes | cited By 1 |
URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85022176113&doi=10.1080%2f09553002.2017.1339918&partnerID=40&md5=64004f974dc2e855714201402fc4e186 |
DOI | 10.1080/09553002.2017.1339918 |
Citation Key | Licursi2017947 |