| Title | Efficient Agrobacterium-based transient expression system for the production of biopharmaceuticals in plants |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2010 |
| Authors | Circelli, P., Donini Marcello, Villani Maria Elena, Benvenuto Eugenio, and Marusic Carla |
| Journal | Bioengineered Bugs |
| Volume | 1 |
| Pagination | 221-224 |
| ISSN | 19491018 |
| Keywords | Agrobacterium, Agrobacterium tumefaciens, artichoke mottled crinckle virus, artichoke mottled crinkle virus, article, Bioengineering, coat protein, controlled study, Cynara scolymus, Gene expression, gene expression system, gene silencing, Genetically Modified, heavy chain, Human immunodeficiency virus 1, human immunoglobulin, Humans, light chain, Nicotiana benthamiana, nonhuman, Pharmaceutical Preparations, plant virus, plant yield component, Plants, Potato virus X, Recombinant Proteins, Rhizobium radiobacter, Tobacco, Tomato bushy stunt virus |
| Abstract | We have recently described an efficient transient expression system mediated by Agrobacterium tumefaciens for the production of HIV-1 Nef protein in Nicotiana benthamiana plants. In order to enhance the yield of recombinant protein we assayed the effect of three gene-silencing viral suppressor proteins (P25 of Potato Virus X, P19 of Artichoke Mottled Crinckle virus and Tomato Bushy Stunt virus) on Nef expression levels. Results demonstrated that AMCV-P19 gave the highest Nef yield (1.3% of total soluble protein) and that this effect was correlated to a remarkable decrease of Nef-specific small interfering RNAs (siRNAs) indicating an effective modulation of RNA silencing mechanisms. Here we report additional data on the production of different heterologous proteins including human immunoglobulin heavy and light chains and a virus coat protein that demonstrate the robustness of this co-agroinfiltration expression system boosted by the AMCV-P19 gene-silencing suppressor. © 2010 Landes Bioscience. |
| Notes | cited By 10 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79851482941&doi=10.4161%2fbbug.1.3.11722&partnerID=40&md5=83e6e92c2326a98558b14a6439ac007c |
| DOI | 10.4161/bbug.1.3.11722 |
| Citation Key | Circelli2010221 |
