Title | Flavonoid metabolism in Forsythia flowers |
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Publication Type | Articolo su Rivista peer-reviewed |
Year of Publication | 1998 |
Authors | Rosati, C., Cadic A., Duron M., Amiot M.-J., Tacchini M., Martens S., and Forkmann G. |
Journal | Plant Science |
Volume | 139 |
Pagination | 133-140 |
ISSN | 01689452 |
Keywords | article, enzyme activity, flavonoid, Forsythia giraldiana, Forsythia intermedia, Forsythia ovata, gene expression regulation, high performance liquid chromatography, metabolism, oxidoreductase, phytochemistry, transgenic plant |
Abstract | The flavonoid pathway metabolism was studied in petals and sepals of Forsythia X intermedia cv. 'Spring Glory'. The activities of the flavonoid enzymes chalcone synthase (CHS), flavanone 3-hydroxylase (FHT) and flavonol synthase (FLS) were measured. The dihydroflavonol 4-reductase (DFR) role was also studied by comparing flavonoid accumulation in transgenic plants for a heterologous DFR gene and wild-type F. X intermedia cv. 'Spring Glory', already investigated for DFR gene expression and activity. HPLC analyses complemented enzymatic investigations, showing that: (i) rutin (quercetin 3- rutinoside) is the major flavonol accumulated in petals and sepals (ca. 90% of the flavonol pool) and; (ii) quercetin and cyanidin derivatives are the exclusive flavonols and anthocyanins in sepals, respectively. The overall data demonstrated that the flavonoid pathway in F. X intermedia flower organs leads to the major accumulation of 3',4'-dihydroxylated compounds, and that 3'-hydroxylation of the B-ring occurs mainly at flavonoid intermediate stage(s). Comparative HPLC analyses of F. X intermedia cv. 'Spring Glory' and three other genotypes (F. giraldiana, F. X intermedia 'Korfor' Goldzauber® and F. ovata 'Robusta') confirmed the major production of flavonols by Forsythia flavonoid metabolism and suggested a method for screening Forsythia genotypes based on anthocyanin accumulation pattern in sepals. |
Notes | cited By 5 |
URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032585632&doi=10.1016%2fS0168-9452%2898%2900180-0&partnerID=40&md5=7d65577ce0c04465c8ce5b2b47b87a11 |
DOI | 10.1016/S0168-9452(98)00180-0 |
Citation Key | Rosati1998133 |