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Two dimensional patterning of fluorescent proteins in hydrogels

TitleTwo dimensional patterning of fluorescent proteins in hydrogels
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2006
AuthorsDi Benedetto, Francesca, Biasco A., Bizzarri R., Arosio D., Ricci F., Beltram F., Cingolani R., and Pisignano D.
JournalLangmuir
Volume22
Pagination29-31
ISSN07437463
KeywordsAequorea victoria, animal, Animals, article, Atomic Force, Atomic force microscopy, Biomedical Engineering, chemical structure, chemistry, Controllable proteins, enhanced green fluorescent protein, fluorescence, fluorescent dye, Fluorescent Dyes, genetics, green fluorescent protein, Green Fluorescent Proteins, hydrogel, Hydrogels, in vitro study, Light emission, Light-emitting biomolecules, Luminescence, Microscopy, Microstructure, Models, Molecular, Photochromic biomolecules, Photodegradation, Proteins, Solid surfaces, Surface properties, surface property, Two dimensional
Abstract

This work describes the successful micropatterning of hybrid systems consisting of hydrogel-dispersed optically active and controllable proteins on solid surfaces without degradation of the photophysical properties of the light-emitting biomolecules. It demonstrates the preservation of the luminescence properties of proteins entrapped into isolated microstructures of poly(acrylamide) gel. This way we can exploit both the structural and function-preserving properties of the hydrogels and the functionality of light-emitting proteins. We believe that this approach can open the way to the realization of nanopatterned optical memories based on photochromic biomolecules. © 2006 American Chemical Society.

Notes

cited By 7

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-30344451104&doi=10.1021%2fla052893k&partnerID=40&md5=d032e18498c89b928b4332e9e0817c15
DOI10.1021/la052893k
Citation KeyDiBenedetto200629