| Title | A new method and tool for detection and quantification of PM oxidative potential |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2015 |
| Authors | Ciriello, F., Gualtieri Maurizio, Longhin E., Ruffo R., Camatini M., and Parenti P. |
| Journal | Environmental Science and Pollution Research |
| Volume | 22 |
| Pagination | 12469-12478 |
| ISSN | 09441344 |
| Keywords | analytical method, atmospheric pollution, chemistry, cytochrome, cytochrome c, Cytochromes c, electrode, Electrodes, Environmental monitoring, Gold, metabolism, oxidation, oxidation reduction reaction, Oxidation-Reduction, particulate matter, phosphine derivative, Phosphines, procedures, quinone derivative, Quinones, reactive oxygen metabolite, Reactive Oxygen Species, redox conditions, spectrophotometry, superoxide, Superoxides, tris(2-carboxyethyl)phosphine |
| Abstract | Airborne particulate matter (PM) contains several quinones, which are able to generate reactive oxygen species impacting on cell viability. A method able to detect and quantify PM oxidative potential, based on the cytochrome c (cyt-c) reduction by means of superoxide anion produced through quinones redox cycling in the presence of reducing agents, is here described. Tris(2-carboxyethyl)phosphine resulted to be the most efficient reducing agent among the ones tested. The procedure included rapid particles extraction, followed by two alternative analytical methods, a spectrophotometric assay based on the initial rate of cyt-c reduction at 550 nm, and an amperometric assay, based on self-assembled monolayers modified gold electrodes. The smallest amount of PM needed to obtain an evaluable signal is 2 μg. The described procedure may represent a starting point to develop devices for PM measurements in polluted atmospheric environments. © 2015, Springer-Verlag Berlin Heidelberg. |
| Notes | cited By 3 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937970231&doi=10.1007%2fs11356-015-4551-2&partnerID=40&md5=38ed8535e9505f05d316c9ddb52ae6f0 |
| DOI | 10.1007/s11356-015-4551-2 |
| Citation Key | Ciriello201512469 |
