| Title | Determination of phenolic acids using Trametes versicolor laccase |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2007 |
| Authors | Odaci, D., Timur S., Pazarlioglu N., Montereali Maria Rita, Vastarella W, Pilloton R., and Telefoncu A. |
| Journal | Talanta |
| Volume | 71 |
| Pagination | 312-317 |
| Keywords | Biosensors, Body fluids, Carboxylic acids, Enzymes, Ferrocenes, Graphite electrodes, Laccase biosensors, pH effects, Phenolic acids, Phenols, Screen-printed electrodes |
| Abstract | Two biosensors based on Trametes versicolor laccase (TvL) were developed for the determination of phenolic compounds. Commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. The systems were calibrated for three phenolic acids. Linearity was obtained in the concentration range 0.1-1.0 μM caffeic acid, 0.05-0.2 μM ferulic acid, 2.0-14.0 μM syringic acid for laccase immobilised on a commercial oxygen electrode and 2.0-30.0 μM caffeic acid, 2.0-10.0 μM ferulic acid, 4.0-30.0 μM syringic acid for laccase immobilised on ferrocene-modified screen-printed electrodes. Furthermore, optimal pH, temperature and thermal stability studies were performed with the commercial oxygen electrode. Both electrodes were used for determination of a class of phenolic acids, achieving a cheap and fast tool and an easy to be used procedure for screening real samples of human plasma. © 2006 Elsevier B.V. All rights reserved. |
| Notes | cited By 31 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33845939370&doi=10.1016%2fj.talanta.2006.04.032&partnerID=40&md5=2bbfc26eb66fc866521ae3590f50a771 |
| DOI | 10.1016/j.talanta.2006.04.032 |
| Citation Key | Odaci2007312 |
