| Title | Bim-dependent apoptosis follows IGFBP-5 down-regulation in neuroblastoma cells. |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2006 |
| Authors | Tanno, Barbara, Vitali Roberta, De Arcangelis Davide, Mancini Camillo, Eleuteri Patrizia, Dominici Carlo, and Raschellà Giuseppe |
| Journal | Biochem Biophys Res Commun |
| Volume | 351 |
| Issue | 2 |
| Pagination | 547-52 |
| Date Published | 2006 Dec 15 |
| ISSN | 0006291X |
| Keywords | Apoptosis, Apoptosis Regulatory Proteins, bcl-2-Associated X Protein, Bcl-2-Like Protein 11, Cell Line, Tumor, Down-Regulation, Extracellular Signal-Regulated MAP Kinases, Humans, Insulin-Like Growth Factor Binding Protein 5, Membrane Proteins, Mitochondria, Neuroblastoma, Proto-Oncogene Proteins |
| Abstract | The insulin-like growth factor (IGF) axis is frequently activated in neuroblastoma (NB) tumors and cell lines. We show that silencing endogenous expression of IGF Binding Protein-5 (IGFBP-5) in NB cells by using microRNA and siRNA causes mitochondrial apoptosis that is characterized by: (a) release of cytochrome C in the cytoplasm and activation of caspase 9; (b) Erk1 and Erk2 inhibition; and (c) upregulation of pro-apoptotic proteins Bim and Bax. Bim upregulation is caused, at least in part, by protein stabilization that may depend on inhibition of Erk1 and Erk2. Of interest, Bim knock-down by siRNA decreases apoptosis in IGFBP-5-interfered cells. Thus, inhibition of endogenously produced IGFBP-5 is associated with Bim-dependent apoptosis in NB cells. |
| Notes | cited By 6 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33750506305&doi=10.1016%2fj.bbrc.2006.10.062&partnerID=40&md5=c432d6d227bcdf4982f9f775d13ad80b |
| DOI | 10.1016/j.bbrc.2006.10.062 |
| Alternate Journal | Biochem. Biophys. Res. Commun. |
| Citation Key | 4917 |
| PubMed ID | 17067554 |
