Title | Inhibition of IL-2 production by Nil-2-a in murine T cells |
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Publication Type | Articolo su Rivista peer-reviewed |
Year of Publication | 1998 |
Authors | Pucci, S., Doria G., Barile S., Pioli Claudio, and Frasca D. |
Journal | International Immunology |
Volume | 10 |
Pagination | 1435-1440 |
ISSN | 09538178 |
Keywords | Age Factors, Aging, animal cell, Animals, article, CD4 antigen, CD4-Positive T-Lymphocytes, cycloheximide, Female, Genetic, Inbred C57BL, interleukin 2, Interleukin-2, lymphocyte activation, Messenger, messenger RNA, Mice, mouse, nonhuman, priority journal, RNA, T lymphocyte, T-Lymphocytes, Time Factors, Transcription, transcription regulation, zinc finger protein, Zinc Fingers |
Abstract | The loss of IL-2 production is the main defect accounting for age-related immunodeficiencies. We have investigated the molecular mechanisms involved in the decrease of IL-2 production in CD4+ T cells from aging mice. Our results demonstrate that the stability of IL-2 mRNA increases in T cells from young mice, whereas it declines in T cells from old mice with the time of stimulation, suggesting the existence of different mechanisms of post-transcriptional regulation in young and old mice. We found that the IL-2 mRNA level in T cells from young but not from old mice increased up to 6- to 10-fold by addition of cycloheximide (CHX) while the stability of IL-2 mRNA is not affected. We then looked for IL-2 inducible inhibitory factors in T cells from young and old mice and demonstrated the presence of Nil-2-a, a zinc finger protein which negatively controls IL-2 gene transcription in human cells. This protein could be detected in T cells from both young and old mice, yet, in the presence of CHX, its binding activity was reduced by 75% in T cells from young but not from old mice. These findings show that Nil-2-a accounts for the negative control of IL-2 production in the mouse and explain the reduced IL-2 production in aging. |
Notes | cited By 5 |
URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031660614&doi=10.1093%2fintimm%2f10.10.1435&partnerID=40&md5=9e2e61ebd5cc3a8a3223dcae5e7ff67d |
DOI | 10.1093/intimm/10.10.1435 |
Citation Key | Pucci19981435 |