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Flow cytometry applications in the evaluation of sperm quality: Semen analysis, sperm function and DNA integrity

TitleFlow cytometry applications in the evaluation of sperm quality: Semen analysis, sperm function and DNA integrity
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2005
AuthorsCordelli, Eugenia, Eleuteri Patrizia, Leter Giorgio, Rescia M., and Spanò M.
JournalContraception
Volume72
Pagination273-279
ISSN00107824
Keywordsabortion, acrosome, andrology, Animals, Apoptosis, Assisted, birth defect, cell viability, chromatin, chromatin structure, Comet Assay, computer analysis, conference paper, cytometry, DNA, DNA determination, embryotoxicity, environmental exposure, Environmental monitoring, Fertility, Flow cytometry, fluorescence, fluorescence in situ hybridization, Fluorescent Dyes, gender, Genomics, human, Humans, In Situ Hybridization, In Situ Nick-End Labeling, male, mammal, mitochondrion, nick end labeling, nonhuman, occupational exposure, progeny, Proteomics, qualitative analysis, Reproductive Techniques, reproductive toxicity, risk, Semen, semen analysis, sperm, Spermatozoa, spermatozoon count, structure analysis, X chromosome, Y chromosome
Abstract

Flow cytometry (FCM) has been extensively used to study mammalian sperm in the areas of reproductive toxicology (to monitor effects from environmental, occupational and therapeutic exposures), veterinary science (to preselect the gender of offspring by sorting X- and Y-chromosome-bearing sperm) and clinical andrology (to assess individual fertility potential). Using FCM, a variety of sperm features can now be rapidly measured on a cell-by-cell basis such as sperm count, viability, acrosomal integrity, mitochondrial function and DNA integrity; the last one is involved in postfertilization failure and embryo toxicity. It is foreseen that only a multiplex approach, which includes FCM assays together with the new genomics/proteomics methods, could increase the predictive power of fertility status and help identify susceptible subpopulations of men at risk for infertility, spontaneous abortions and birth defects. © 2005 Elsevier Inc. All rights reserved.

Notes

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-25144525484&doi=10.1016%2fj.contraception.2005.03.004&partnerID=40&md5=a831990c2d4c42a73939aac3e55aab86
DOI10.1016/j.contraception.2005.03.004
Citation KeyCordelli2005273