| Title | HPLC screening of natural vitamin E from mediterranean plant biofactories - A basic tool for pilot-scale bioreactors production of α-tocopherol |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2005 |
| Authors | Sivakumar, G., Bacchetta Loretta, Gatti Rosanna, and Zappa Giovanna |
| Journal | Journal of Plant Physiology |
| Volume | 162 |
| Pagination | 1280 - 1283 |
| Date Published | 2005/// |
| Keywords | Antioxidant, Biofactory, Bioreactor, Hazelnut, Nutraceutical, α-tocopherol |
| Abstract | The study was performed in order to investigate a simple, efficient, reliable and rapid method of extracting and quantifying natural vitamin E for pressurized liquid extraction (PLE) as well as high-performance liquid chromatography (HPLC) analysis. Lyophilized Corylus avellana L. nut samples were powdered by high-speed milling with Waring blender for 40 s. α-Tocopherol was extracted from the nut tissue powder using dehydrated hexane fortified with 0.01% butylated hydroxytoluene (BHT, co-antioxidant). The rate of α-tocopherol accumulation showed differences among nut samples collected in different areas of Italy. Sarda Piccola nut biofactory contained higher amount (81.17 μg/g d.w) of α-tocopherol than other-local eleven Italian cultivar nuts. These results provide insight into the biofactory basis for α-tocopherol accumulation in hazelnut and give the suitable cultivar tissues to establish pilot-scale bioreactors production of natural bioactive vitamin E. © 2005 Elsevier GmbH. All rights reserved. |
| Notes | Cited By (since 1996): 5Export Date: 1 September 2010Source: Scopus |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-27344445009&partnerID=40&md5=c3bab97ecf2f6eee25379023e4ab955b |
| Citation Key | 542 |
