Sorry, you need to enable JavaScript to visit this website.

Hairpin RNA-mediated silencing of Plum pox virus P1 and HC-Pro genes for efficient and predictable resistance to the virus

TitleHairpin RNA-mediated silencing of Plum pox virus P1 and HC-Pro genes for efficient and predictable resistance to the virus
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2005
AuthorsDi Nicola-Negri, Elisa, Brunetti A., Tavazza Mario, and Ilardi Vincenza
JournalTransgenic Research
Volume14
Pagination989 - 994
Date Published2005///
KeywordsPathogen-derived resistance, Potyvirus, RNAi, Silencing suppressor, siRNA
Abstract

We report the application of the hairpin-mediated RNA silencing technology for obtaining resistance to Plum pox virus (PPV) infection in Nicotiana benthamiana plants. Four sequences, covering the P1 and silencing suppressor HC-Pro genes of an Italian PPV M isolate, were introduced into N. benthamiana plants as two inverted repeats separated by an intron sequence under the transcriptional control of the Cauliflower Mosaic Virus 35S promoter. In a leaf disk infection assay, 38 out of 40 T0 transgenic plants were resistant to PPV infection. Eight lines, 2 for each construct, randomly selected among the 38 resistant plants were further analysed. Two hundred forty eight out of 253 T1 transgenic plants were resistant to local and systemic PPV infection. All transgenic single locus lines were completely resistant. These data indicate that the RNA silencing of PPV P1/HCPro sequences results in an efficient and predictable PPV resistance, which may be utilized in obtaining stone fruit plants resistant to the devastating Sharka disease. © Springer 2005.

Notes

Cited By (since 1996): 22Export Date: 25 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-28244479938&partnerID=40&md5=83bad0c3a9bcd6a93c7aecde24f83082
Citation Key282