| Title | CD4+CD25lowGITR+ cells: A novel human CD4+ T-cell population with regulatory activity |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2011 |
| Authors | Bianchini, Rodolfo, Bistoni Onelia, Alunno Alessia, Petrillo Maria Grazia, Ronchetti Simona, Sportoletti Paolo, Bocci Elena Bartoloni, Nocentini Giuseppe, Gerli Roberto, and Riccardi Carlo |
| Journal | European Journal of Immunology |
| Volume | 41 |
| Pagination | 2269 – 2278 |
| Type of Article | Article |
| ISSN | 15214141 |
| Keywords | adult, animal cell, Animals, Antigens, article, blood, CD, CD4+ CD25+ T lymphocyte, CD4+ T lymphocyte, CD4-Positive T-Lymphocytes, cell function, cell proliferation, controlled study, cytotoxic T lymphocyte antigen 4, Flow cytometry, Gene expression, glucocorticoid induced tumor necrosis factor receptor, Glucocorticoid-Induced TNFR-Related Protein, human, human cell, Humans, Immunologic Memory, Immunomodulation, Immunophenotyping, immunoregulation, interleukin 10, interleukin 2 receptor alpha, interleukin 7 receptor, Interleukin-10, Interleukin-2 Receptor alpha Subunit, lymphocyte proliferation, memory T lymphocyte, Mice, Middle Aged, nonhuman, priority journal, protein expression, protein function, Regulatory, regulatory T lymphocyte, Reverse Transcriptase Polymerase Chain Reaction, T lymphocyte activation, T lymphocyte subpopulation, T-Lymphocyte Subsets, T-Lymphocytes, transcription factor FOXP3, transforming growth factor beta, young adult |
| Abstract | Treg subsets play a role in sustaining peripheral tolerance, are characterized by markers such as forkhead winged-helix transcription factor (FOXP3) and CD25, and produce suppressive cytokines, such as IL-10 and TGF-β. Glucocorticoid-induced TNF receptor family-related (GITR) protein has been suggested to regulate Treg activity in mice. The aim of our study was to investigate GITR expression in human CD4+ T lymphocytes and its possible role in Treg function. Results indicate that a subset of CD4+ T cells in the peripheral blood expresses GITR and low levels of CD25 (CD4+CD25lowGITR+). These cells show Treg features as they express FOXP3, IL-10, TGF-β and are anergic but, as opposed to natural Tregs, express low levels of CTLA-4 and are CD127high. CD4+CD25lowGITR+ cells represent a low percentage of the CD4+ T-cell population (0.32-1.74%) and are mostly memory cells. Functional experiments demonstrated that CD4+CD25lowGITR+ cells have relevant suppressive activity that depends on TGF-β. Moreover, an anti-GITR Ab inhibited their suppressive activity, as observed in CD4+CD25+ murine Tregs. Taken together, these data indicate that human CD4+CD25lowGITR+ cells represent a distinct Treg subpopulation. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| Notes | Cited by: 53; All Open Access, Bronze Open Access |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960718028&doi=10.1002%2feji.201040943&partnerID=40&md5=63b6f207b19f780e077221ca7ae91264 |
| DOI | 10.1002/eji.201040943 |
| Citation Key | Bianchini20112269 |
| PubMed ID | 21557210 |
