| Title | Glucocorticoid-Induced TNFR family Related gene (GITR) enhances dendritic cell activity |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2011 |
| Authors | Ronchetti, Simona, Nocentini Giuseppe, Petrillo Maria Grazia, Bianchini Rodolfo, Sportoletti Paolo, Bastianelli Alessandra, Ayroldi Emira M., and Riccardi Carlo |
| Journal | Immunology Letters |
| Volume | 135 |
| Pagination | 24 – 33 |
| Type of Article | Article |
| ISSN | 01652478 |
| Keywords | animal cell, Animal cell culture, animal experiment, animal tissue, Animals, article, bone marrow cell, Bone Marrow Cells, CD4+ CD25+ T lymphocyte, cell activity, coculture, controlled study, cytokine production, Cytokines, dendritic cell, Dendritic Cells, glucocorticoid induced tumor necrosis factor receptor, Glucocorticoid-Induced TNFR-Related Protein, Inbred BALB C, interleukin 10, interleukin 6, Knockout, lymphocyte activation, Lymphocyte Count, lymphocyte proliferation, Mice, mouse, Nerve Growth Factor, nonhuman, priority journal, protein expression, protein function, Receptors, Regulatory, T lymphocyte activation, T-Lymphocytes, transforming growth factor beta, Tumor Necrosis Factor |
| Abstract | Glucocorticoid-Induced TNFR family Related gene (GITR), a Tumor Necrosis Factor Receptor Superfamily (TNFRSF) member involved in immune/inflammatory processes, has been previously shown to regulate T cell activation. To study GITR role in antigen presenting cells, we evaluated the capability of bone marrow derived dendritic cells (BMDC) from GITR -/- mice to stimulate the activation of CD4 +CD25 - T lymphocytes. We found that GITR -/- BMDC are weaker stimulators of T cell proliferation than GITR +/+ BMDC, either in syngenic or allogenic BMDC/T cell co-cultures. Expression of GITR in GITR -/- BMDC restored their ability to activate T cells while GITR silencing in GITR +/+ BMDC inhibited the capability to stimulate T cells. GITR -/- BMDC showed a reduced production of the pro-inflammatory cytokine IL-6 and an increased production of the anti-inflammatory cytokine IL-10. Notably, co-culture of CD4 +CD25 - cells with GITR -/- BMDC originated FoxP3 + cells, secreting IL-10 and TGF-β. Finally, in vivo injection of GITR -/- OVA-loaded BMDC led to a lower cell number and a lower activated cell number in draining lymph nodes than in GITR +/+ OVA-loaded BMDC injected mice. Together, these results indicate that GITR plays a role in regulating BMDC activity. © 2010 Elsevier B.V. |
| Notes | Cited by: 15 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-78751705229&doi=10.1016%2fj.imlet.2010.09.008&partnerID=40&md5=fa9188521174bdf130711ea17c86daba |
| DOI | 10.1016/j.imlet.2010.09.008 |
| Citation Key | Ronchetti201124 |
| PubMed ID | 20883723 |
