Title | Unsaturated fatty acids-dependent linkage between respiration and fermentation revealed by deletion of hypoxic regulatory KlMGA2 gene in the facultative anaerobe-respiratory yeast Kluyveromyces lactis |
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Publication Type | Articolo su Rivista peer-reviewed |
Year of Publication | 2015 |
Authors | Ottaviano, Daniela, Montanari Arianna, De Angelis Lorenzo, Santomartino Rosa, Visca Andrea, Brambilla Luca, Rinaldi Teresa, Bello Cristiano, Reverberi Massimo, and Bianchi Michele M. |
Journal | FEMS Yeast Research |
Volume | 15 |
Pagination | fov028 |
ISSN | 15671356 |
Keywords | antifungal agent, Antifungal Agents, Antimycin A, antimycin A1, article, bacterial protein, Bacterial Proteins, cardiolipin, Cardiolipins, cell function, cell hypoxia, cell structure, cold shock response, cold stress, Cold-Shock Response, cytoplasm, drug effects, ergosterol, fatty acid, Fatty acids, Fermentation, Fungal, fungal gene, fungal strain, gene deletion, gene expression regulation, Genetic, genetic transcription, genetics, Glucose, glycolysis, hypoxia, klmga 2 gene, Kluyveromyces, Kluyveromyces lactis, membrane protein, Membrane Proteins, metabolism, Mitochondria, mitochondrial respiration, mitochondrion, nonhuman, Oxygen consumption, Phenotype, physiology, regulator gene, Transcription, transcription factor, Transcription Factors, Unsaturated, unsaturated fatty acid |
Abstract | In the yeast Kluyveromyces lactis, the inactivation of structural or regulatory glycolytic and fermentative genes generates obligate respiratory mutants which can be characterized by sensitivity to the mitochondrial drug antimycin A on glucose medium (Rag- phenotype). Rag- mutations can occasionally be generated by the inactivation of genes not evidently related to glycolysis or fermentation. One such gene is the hypoxic regulatory gene KlMGA2. In this work, we report a study of the many defects, in addition to the Rag- phenotype, generated by KlMGA2 deletion. We analyzed the fermentative and respiratory metabolism, mitochondrial functioning and morphology in the Klmga2 strain. We also examined alterations in the regulation of the expression of lipid biosynthetic genes, in particular fatty acids, ergosterol and cardiolipin, under hypoxic and cold stress and the phenotypic suppression by unsaturated fatty acids of the deleted strain. Results indicate that, despite the fact that the deleted mutant strain had a typical glycolytic/fermentative phenotype and KlMGA2 is a hypoxic regulatory gene, the deletion of this gene generated defects linked to mitochondrial functions suggesting new roles of this protein in the general regulation and cellular fitness of K. lactis. Supplementation of unsaturated fatty acids suppressed or modified these defects suggesting that KlMga2 modulates membrane functioning or membrane-associated functions, both cytoplasmic and mitochondrial. © FEMS 2015. |
Notes | cited By 14 |
URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949225086&doi=10.1093%2ffemsyr%2ffov028&partnerID=40&md5=8d8033aaa2836735e9649b9c8e76cfd4 |
DOI | 10.1093/femsyr/fov028 |
Citation Key | ottaviano2015unsaturated |