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Analysis of the heat-shock protein pattern during somatic embryogenesis of carrot

TitleAnalysis of the heat-shock protein pattern during somatic embryogenesis of carrot
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1983
AuthorsPitto, L., Schiavo F.L., Giuliano Giovanni, and Terzi M.
JournalPlant Molecular Biology
Volume2
Pagination231-237
ISSN01674412
Abstract

The pattern of proteins synthesized by carrot cells after heat-shock was analyzed by SDS-polyacrylamide gel electrophoresis. A study was made of the time of appearance and disappearance of the heat-shock bands and a comparison was made of the heat-shock proteins present in cell extracts obtained from cell suspensions, callus, protoplasts and the different stages of somatic embryogenesis. From this comparison a number of differences and hence of stage-specific markers could be seen. This type of analysis was also carried out in the presence of α-amanitin in order to get information on the type of control of heat-shock protein synthesis. It turned out that in all stages after the initial globular stage, α-amanitin does not alter the heat-shock protein pattern suggesting pre-existence of the relative mRNA's. © 1983 Martinus Nijhoff/Dr W. Junk Publishers.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0001190256&doi=10.1007%2fBF01578641&partnerID=40&md5=c796c61f40308b8769ada01e06bf3085
DOI10.1007/BF01578641
Citation KeyPitto1983231