| Title | Gene expression profiling of A549 cells exposed to Milan PM2.5 |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2012 |
| Authors | Gualtieri, Maurizio, Longhin E., Mattioli M., Mantecca P., Tinaglia V., Mangano E., Proverbio M.C., Bestetti G., Camatini M., and Battaglia C. |
| Journal | Toxicology Letters |
| Volume | 209 |
| Pagination | 136-145 |
| ISSN | 03784274 |
| Keywords | antigen, Aromatic, article, Aryl Hydrocarbon Hydroxylases, Blotting, Cell Line, Cell Survival, cell viability, Comet Assay, controlled study, cytochrome P450 1A1, cytochrome P450 1B1, Cytotoxicity, DNA, DNA damage, DNA strand breakage, environmental exposure, epiregulin, Epithelial Cells, Flow cytometry, FOS like antigen1, Gene expression, gene expression profiling, human, human cell, Humans, in vitro study, intracellular signaling, Italy, microarray analysis, Oligonucleotide Array Sequence Analysis, particulate matter, Polycyclic Hydrocarbons, priority journal, reactive oxygen metabolite, Reactive Oxygen Species, real time polymerase chain reaction, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, RNA, seasonal variation, Seasons, signal transduction, summer, unclassified drug, upregulation, Urban Population, Western, winter |
| Abstract | Background: Particulate matter (PM) has been associated to adverse health effects in exposed population and DNA damage has been extensively reported in in vitro systems exposed to fine PM (PM2.5). The ability to induce gene expression profile modulation, production of reactive oxygen species (ROS) and strand breaks to DNA molecules has been investigated in A549 cells exposed to winter and summer Milan PM2.5. Results: A549 cells, exposed to 10μg/cm 2 of both winter and summer PM2.5, showed increased cytotoxicity at 24h and a significant increase of ROS at 3h of treatment. Despite these similar effects winter PM induced a higher number of gene modulation in comparison with summer PM. Both PMs modulated genes related to the response to xenobiotic stimuli (CYP1A1, CYP1B1, TIPARP, ALDH1A3, AHRR) and to the cell-cell signalling (GREM1) pathways with winter PM2.5 inducing higher fold increases. Moreover the winter fraction modulated also JUN (cell-cell signalling), GDF15, SIPA1L2 (signal transduction), and HMOX1 (oxidative stress). Two genes, epiregulin (EREG) and FOS-like antigen1 (FOSL1), were significantly up-regulated by summer PM2.5. The results obtained with the microarray approach have been confirmed by qPCR and by the analysis of CYP1B1 expression. Comet assay evidenced that winter PM2.5 induced more DNA strand breaks than the summer one. Conclusion: Winter PM2.5 is able to induce gene expression alteration, ROS production and DNA damage. These effects are likely to be related to the CYP enzyme activation in response to the polycyclic aromatic hydrocarbons (PAHs) adsorbed on particle surface. © 2011 Elsevier Ireland Ltd. |
| Notes | cited By 42 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855513089&doi=10.1016%2fj.toxlet.2011.11.015&partnerID=40&md5=575240a3a6a3c8bc333c9381d51ca167 |
| DOI | 10.1016/j.toxlet.2011.11.015 |
| Citation Key | Gualtieri2012136 |
