| Title | An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 1988 |
| Authors | Giuliano, Giovanni, Pichersky E., Malik V.S., Timko M.P., Scolnik P.A., and Cashmore A.R. |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 85 |
| Pagination | 7089-7093 |
| ISSN | 00278424 |
| Keywords | article, Base Sequence, DNA binding protein, DNA-Binding Proteins, Genes, genetics, homeobox, molecular genetics, Molecular Sequence Data, nucleotide sequence, Plant, Plants, protein binding, Ribulose-Bisphosphate Carboxylase, ribulosebisphosphate carboxylase, vegetable, Vegetables |
| Abstract | A protein factor, identified in nuclear extracts obtained from tomato (Lycopersicon esculentum, Solanaceae) and Arabidopsis thaliana (Brassicaceae) seedlings, specifically binds upstream sequences from the plant light-regulated gene family encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (RBCS). RBCS upstream sequences from tomato, pea (Pisum sativum, Leguminosae), and Arabidopsis are recognized by the factor. The factor recognition occurs via a short conserved sequence (G box) whose consensus sequence is 5'-TCTTACACGTGGCAYY-3' (where Y is pyrimidine). This sequence is distinct from the GT motif described previously in RBCS promoters. Two other conserved sequences, showing a lesser degree of evolutionary conservation, are found upstream of the G box but do not bind to the G box binding factor (GBF). Twelve nucleotides within the G box are sufficient for the formation of a stable DNA-GBF complex. GBF is found in both light-grown and dark-adapted tomato leaf extracts, but it is present in greatly reduced amounts in root extracts. |
| Notes | cited By 447 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024094933&doi=10.1073%2fpnas.85.19.7089&partnerID=40&md5=20e3e9190b6ebc3c306ebf2fc2353fb1 |
| DOI | 10.1073/pnas.85.19.7089 |
| Citation Key | Giuliano19887089 |
